Mass Spectrometry Based Serum Proteomics
publications
book chapter
I really like Methods in Molecular Biology book series. They publish step by step protocols with detailed information on materials and methods to carry out the experiment in a reproducible manner. In particular notes section provides useful tip and troubleshooting guide.
We published a protocol entitled Mass Spectrometry-Based Serum Proteomics for Biomarker Discovery and Validation. It presents a workflow detailing sample preparation with and without immunodepletion of high abundant serum/plasma proteins and, LC-MS/MS methodology for discovery and targeted measurements of candidate biomarkers.